Synthetic sialylglycopolymer receptor for virus detection using cantilever-based sensors.
Identifieur interne : 000D02 ( Main/Exploration ); précédent : 000D01; suivant : 000D03Synthetic sialylglycopolymer receptor for virus detection using cantilever-based sensors.
Auteurs : P V Gorelkin [Russie] ; A S Erofeev ; G A Kiselev ; D V Kolesov ; E V Dubrovin ; I V YaminskySource :
- The Analyst [ 1364-5528 ] ; 2015.
Descripteurs français
- KwdFr :
- MESH :
- isolement et purification : Virus de la grippe A.
- métabolisme : Récepteurs artificiels, Virus de la grippe A.
- Fétuines, Microscopie à force atomique, Oligosaccharides, Récepteurs artificiels, Résines acryliques.
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Acrylic Resins, Fetuins, Oligosaccharides, Receptors, Artificial.
- isolation & purification : Influenza A virus.
- metabolism : Influenza A virus, Receptors, Artificial.
- Microscopy, Atomic Force.
Abstract
We describe the rapid, label-free detection of Influenza A viruses using a cantilever transducer modified with a synthetic sialylglycopolymer receptor layer. Surface stresses induced by viruses binding to the receptor layer were used as the analytical signal. The synthetic sialylglycopolymer receptor layer can be used in nanoscale strain-gauge cantilever transducers for highly sensitive virus detection. Strain-gage transducers using such sensor layers exhibit long lifetimes, high sensitivities, and possible regeneration. Nanomechanical cantilever systems using optical detectors were used for the surface stress measurements. We demonstrated the positive, label-free detection of Influenza A at concentrations below 10(6) viruses per ml. In contrast to hemagglutination assays, cantilever sensors are label free, in situ, and rapid (less than 30 min), and they require minimal or nearly no sample preparation.
DOI: 10.1039/c5an01102g
PubMed: 26215598
Affiliations:
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Le document en format XML
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V" uniqKey="Kolesov D" first="D V" last="Kolesov">D V Kolesov</name></author><author><name sortKey="Dubrovin, E V" sort="Dubrovin, E V" uniqKey="Dubrovin E" first="E V" last="Dubrovin">E V Dubrovin</name></author><author><name sortKey="Yaminsky, I V" sort="Yaminsky, I V" uniqKey="Yaminsky I" first="I V" last="Yaminsky">I V Yaminsky</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2015">2015</date><idno type="RBID">pubmed:26215598</idno><idno type="pmid">26215598</idno><idno type="doi">10.1039/c5an01102g</idno><idno type="wicri:Area/PubMed/Corpus">000415</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000415</idno><idno type="wicri:Area/PubMed/Curation">000414</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000414</idno><idno type="wicri:Area/PubMed/Checkpoint">000391</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" 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119991</wicri:regionArea><wicri:noRegion>119991</wicri:noRegion></affiliation></author><author><name sortKey="Erofeev, A S" sort="Erofeev, A S" uniqKey="Erofeev A" first="A S" last="Erofeev">A S Erofeev</name></author><author><name sortKey="Kiselev, G A" sort="Kiselev, G A" uniqKey="Kiselev G" first="G A" last="Kiselev">G A Kiselev</name></author><author><name sortKey="Kolesov, D V" sort="Kolesov, D V" uniqKey="Kolesov D" first="D V" last="Kolesov">D V Kolesov</name></author><author><name sortKey="Dubrovin, E V" sort="Dubrovin, E V" uniqKey="Dubrovin E" first="E V" last="Dubrovin">E V Dubrovin</name></author><author><name sortKey="Yaminsky, I V" sort="Yaminsky, I V" uniqKey="Yaminsky I" first="I V" last="Yaminsky">I V Yaminsky</name></author></analytic><series><title level="j">The Analyst</title><idno type="eISSN">1364-5528</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acrylic Resins (chemistry)</term><term>Fetuins (chemistry)</term><term>Influenza A virus (isolation & purification)</term><term>Influenza A virus (metabolism)</term><term>Microscopy, Atomic Force</term><term>Oligosaccharides (chemistry)</term><term>Receptors, Artificial (chemistry)</term><term>Receptors, Artificial (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Fétuines ()</term><term>Microscopie à force atomique</term><term>Oligosaccharides ()</term><term>Récepteurs artificiels ()</term><term>Récepteurs artificiels (métabolisme)</term><term>Résines acryliques ()</term><term>Virus de la grippe A (isolement et purification)</term><term>Virus de la grippe A (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Acrylic Resins</term><term>Fetuins</term><term>Oligosaccharides</term><term>Receptors, Artificial</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Influenza A virus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Virus de la grippe A</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Influenza A virus</term><term>Receptors, Artificial</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Récepteurs artificiels</term><term>Virus de la grippe A</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Microscopy, Atomic Force</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Fétuines</term><term>Microscopie à force atomique</term><term>Oligosaccharides</term><term>Récepteurs artificiels</term><term>Résines acryliques</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We describe the rapid, label-free detection of Influenza A viruses using a cantilever transducer modified with a synthetic sialylglycopolymer receptor layer. Surface stresses induced by viruses binding to the receptor layer were used as the analytical signal. The synthetic sialylglycopolymer receptor layer can be used in nanoscale strain-gauge cantilever transducers for highly sensitive virus detection. Strain-gage transducers using such sensor layers exhibit long lifetimes, high sensitivities, and possible regeneration. Nanomechanical cantilever systems using optical detectors were used for the surface stress measurements. We demonstrated the positive, label-free detection of Influenza A at concentrations below 10(6) viruses per ml. In contrast to hemagglutination assays, cantilever sensors are label free, in situ, and rapid (less than 30 min), and they require minimal or nearly no sample preparation. </div></front></TEI><affiliations><list><country><li>Russie</li></country></list><tree><noCountry><name sortKey="Dubrovin, E V" sort="Dubrovin, E V" uniqKey="Dubrovin E" first="E V" last="Dubrovin">E V Dubrovin</name><name sortKey="Erofeev, A S" sort="Erofeev, A S" uniqKey="Erofeev A" first="A S" last="Erofeev">A S Erofeev</name><name sortKey="Kiselev, G A" sort="Kiselev, G A" uniqKey="Kiselev G" first="G A" last="Kiselev">G A Kiselev</name><name sortKey="Kolesov, D V" sort="Kolesov, D V" uniqKey="Kolesov D" first="D V" last="Kolesov">D V Kolesov</name><name sortKey="Yaminsky, I V" sort="Yaminsky, I V" uniqKey="Yaminsky I" first="I V" last="Yaminsky">I V Yaminsky</name></noCountry><country name="Russie"><noRegion><name sortKey="Gorelkin, P V" sort="Gorelkin, P V" uniqKey="Gorelkin P" first="P V" last="Gorelkin">P V Gorelkin</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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